NAD+ 1000mg – cellular energy and longevity

What is NAD+?

NAD+ helps cells produce and manage their energy more efficiently and better cope with the "stress" of workload and aging by providing them a key building block for their internal processes.

In a biomedical context the molecule belongs to the pyridine-nucleotide coenzymes and participates directly in redox signaling/metabolic pathways, where it alternates between oxidized and reduced forms (NAD/NADH) and thus links energy metabolism with cellular signaling (overview: Wikipedia: Nicotinamide adenine dinucleotide).

Mechanism of action (research framework)

NAD+ is an essential electron carrier for glycolysis, the Krebs cycle and oxidative phosphorylation; the NAD⁺/NADH ratio influences metabolic flux and mitochondrial function. Besides the redox role, NAD+ is a substrate for NAD-dependent enzymes (e.g., sirtuins and PARP), which regulate transcription, DNA repair and cellular adaptation to stress. This places the peptide at the center of axes such as SIRT1–PGC-1α and the PARP-mediated response to DNA damage (source: PubMed Central (review articles on NAD+).

Preclinical/clinical data

Note: Published human data are mostly for precursors (NR/NMN), and not for directly applied NAD+. According to official sources, translating effects to exogenous NAD+ requires careful experimental design (sources: NEJM, PubMed).

Context of scientific use

In the laboratory environment NAD+ is used as a standard/coenzyme in enzyme activities (dehydrogenases), in tests for mitochondrial function, PARP/sirtuin activities, calibrations for LC–MS/UV and as a control in models of metabolic stress. In the clinical/translational setting it is more often monitored as a biomarker (NAD metabolome) and in interventions with precursors, due to limitations in the stability and cellular transport of the molecule itself.

Pharmacokinetics (for experimental design)

As a polar dinucleotide, exogenous NAD+ has limited membrane permeability; in cellular experiments conditions for transport/hydrolysis (e.g., ectoenzymes) are usually planned and intracellular pools are measured by LC–MS. In in vivo models it is critical to define the matrix (blood/tissue), sampling time and analytical method, since metabolism to nicotinamide/nucleotides can dominate the observed signal.

Purity and specification

NAD Plus from MyPeptid provides a reliable basis for reproducible biochemical assays, as it has a purity >99% (HPLC) and batch control, which minimizes background signals in enzyme and mass-spectrometric analyses.

Formula: C21H27N7O14P2

For sale for educational and research purposes only and is not intended for diagnostic, therapeutic or clinical use.